Assessing the Efficacy and Feasibility of Conditioned Medium as a Culture Reagent for Tumor Organoid Culture: A Comprehensive Evaluation

Abstract

Tumor organoids are three-dimensional models of tumor cells grown in vitro which derived from primary tumors of patients. Tumor organoids highly mimic the characteristics of the primary tumors, retain tumor heterogeneity, and can be used for functional testing in vitro, including high-throughput drug screening and personalized therapeutic strategies. However, the establishment of tumor organoids facing such dilemma as low success rate and growth rate. The use of appropriate and effective culture reagent is pivotal to the success of tumor organoids culture. Nowadays, the most commonly and long-lasting used media in cell culture is conditioned medium, while its application in organoids culture has been controversial due to undefined components. In response, our study conducts a comprehensive analysis, offering compelling evidence supporting the efficacy of conditioned medium in promoting organoids growth and expansion. This research aims to address uncertainties surrounding its use and contribute valuable insights to enhance tumor organoids culture practices.